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Dianova公司为德国的病理免疫组化产品供应商，具备的抗体制备工艺及严格的质控系统，产品在上享有良好的声誉。

克隆号为H09的IDH1（isocitrate dehydrogenase 1, 异柠檬酸盐脱氢酶1）鼠单克隆抗体为Dianova公司近期推出的产品之一，此抗体可特异性的检测脑组织肿瘤中IDH1基因的R132H点突变。IDH1基因的第132密码子的杂合突变是II 和III级神经胶质瘤（根据WHO的肿瘤学分类）的频发事件。在大约70％的星形胶质细胞瘤和少突神经胶质细胞瘤可发现IDH1基因的R132H点突变。

IDH1基因R132H突变在部分脑肿瘤中的频发现象使克隆号为H09的IDH1抗体可高灵敏性和高特异性的用于此类肿瘤的鉴别诊断中：如从原发性胶质母细胞瘤中区分出间变性星形细胞瘤、从毛细胞型星形细胞瘤或室管膜（细胞）瘤中区分出弥漫性星形细胞瘤（WHO分类II级），也可以用于具有IDH1突变的肿瘤细胞浸润前沿的与反应性神经胶质增多症的区分。因此，由于其高灵敏性和高特异性，此抗体在脑部肿瘤的分类分级中以及在检测单一或少量浸润肿瘤细胞时是非常有用的。

DIA-W09 – Anti-IDH1 (Hu) aus Ratte (Klon: W09) – unkonj. – 500 µl

Art.-Nr.                        DIA-W09

Spezifität                      IDH1

Spezies-Reaktivität             Human

Wirtsspezies                     Ratte

Isotyp                           IgG2a

Klon                             W09

Klonalität (Mono-/Polyklonal)     monoklonal

Anwendung                        Western Blot

                                 Immunhistochemie (IHC)
                                 Immunhistochemie (Paraffingewebe)

Konjugation                      unkonjugiert

Format                           ProteinA/G-gereinigt (aus Kulturüberstand)

                                 Lyophilisat

Produktlinie / Thema             Neurobiologie

                                 Tumormarker/-biologie
                                 Immunhistochemie

Zweckbestimmung                  nur für Forschungszwecke

Suchcode                          DIA W09

                                  FFPE
                                  Paraffin

Hersler / Marke                dianova

Reactivity

Isocitrate dehydrogenase (IDH) is an important enzyme in the citric acid cycle and catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate and CO2 while converting NAD+ to NADH. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximay 70% of astrocytomas and oligodendroglial tumors. Mouse antibody clone H09 (product number #DIA-H09)reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Therefore this antibody is highly useful for tumor classification. Rat antibody clone W09 detects both wild type (wt) and point mutated IDH1 in glioblastoma (anti-pan-IDH1) and serves as control for the IDH1 point mutation specific mouse antibody clone H09. Moreover, Tan et al. (Mol Cell Proteomics, 2011) have identified IDH1 as a potential diagnostic and prognostic biomarker for Non-small-cell Lung Cancer (NSCLC). These findings suggest that anti-IDH1wt antibody could be used as a histochemical biomarker for prognosis prediction of NSCLC.

Immunohistochemistry of human IDH1 R132H and human IDH1 wild-type in formalin-fixed paraffinembedded glioblastoma.

A: No reaction of IDH1 R132H mutation specific antibody clone H09 with glioblastoma (200x)
B: Strong reaction of anti-pan-IDH1 specific antibody clone W09 with the same glioblastoma (200x)
C: No reaction of IDH1 R132H mutation specific antibody clone H09 with glioblastoma (400x)
D: Strong reaction of anti-pan-IDH1 specific antibody clone W09 with the same glioblastoma (400x)

A

B

C

D

(pictures courtesy of Prof. Dr. med. Andreas von Deimling, Department of Neuropathology, University Heidelberg / Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany)

References

• Capper D, Weissert S, Balss J, Habel A, Meyer J, Jäger D, Ackermann U, Tessmer C, Korshunov A, Zentgraf H, Hartmann C, von Deimling A. Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol. 20(1): 245-254, 2010

Related References

• Tan F, Jiang Y, Sun N, Chen Z, Lv Y, Shao K, Li N, Qiu B, Gao Y, Li B, Tan X, Zhou F, Wang Z, Ding D, Wang J, Sun J,Hang J, Feng X, Shi S, He F, He J.. Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non small-cell Lung Cancer by Proteomic Analysis. Mol Cell Proteomics 2011 Nov 7. (Epub ahead of print)

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