世界*实验材料供应商 Dianova 正式授权上海起发为其中国代理， Dianova 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海起发一直秉承为中国科研客户带来的产品，的服务，签约 Dianova 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 Dianova 中国代理, Dianova 上海代理， Dianova 北京代理，Dianova 广东代理， Dianova 江苏代理Dianova 湖北代理,Dianova 天津,Dianova 黑龙江代理,Dianova 内蒙古代理,Dianova 吉林代理,Dianova 福建代理, Dianova 江苏代理, Dianova 浙江代理, Dianova 四川代理,

Dianova公司为德国的病理免疫组化产品供应商，具备的抗体制备工艺及严格的质控系统，产品在上享有良好的声誉。

克隆号为H09的IDH1（isocitrate dehydrogenase 1, 异柠檬酸盐脱氢酶1）鼠单克隆抗体为Dianova公司近期推出的产品之一，此抗体可特异性的检测脑组织肿瘤中IDH1基因的R132H点突变。IDH1基因的第132密码子的杂合突变是II 和III级神经胶质瘤（根据WHO的肿瘤学分类）的频发事件。在大约70％的星形胶质细胞瘤和少突神经胶质细胞瘤可发现IDH1基因的R132H点突变。

IDH1基因R132H突变在部分脑肿瘤中的频发现象使克隆号为H09的IDH1抗体可高灵敏性和高特异性的用于此类肿瘤的鉴别诊断中：如从原发性胶质母细胞瘤中区分出间变性星形细胞瘤、从毛细胞型星形细胞瘤或室管膜（细胞）瘤中区分出弥漫性星形细胞瘤（WHO分类II级），也可以用于具有IDH1突变的肿瘤细胞浸润前沿的与反应性神经胶质增多症的区分。因此，由于其高灵敏性和高特异性，此抗体在脑部肿瘤的分类分级中以及在检测单一或少量浸润肿瘤细胞时是非常有用的。

DIA-808 – Anti-CD8a (Ms) from Rat (Clone: GHH8) for mouse FFPE tissue – 200 µl

Art.-Nr.                          DIA-808

Specificity                       CD8a (MHC I Co-Receptor, cytotox. T-cells)

Species Reactivity                Mouse

Host Species                       Rat

Isotype                            IgG2a/k

Clone                              GHH8

Clonality (Mono-/Polyclonal)       monoclonal

Application                       Immunohistochemistry (Paraffin-embedded Sections)

Conjugation                        unconjugated

Format                            lyophilisate

                                  antibody purified (from culture supernatant)

Product line / Topic               CD markers

                                   immunohistochemistry

Intended Use                        for Research Use Only

Search Code                         DIA808

Manufacturer / Brand                dianova

Reactivity:

Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic/suppressor T-cells in murine FFPE tissue by IHC.
CD8 is a marker for T-cells with suppressor and cytotoxic activity. CD8 is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heter-omeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins.
Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.

Immunohistochemistry of mouse CD8a in formalin-fixed paraffin-embedded tissue sections

A, Mouse spleen: The monoclonal antibody clone GHH8 specifically stains CD8a in standard formalin-fixed paraffin-embedded mouse spleen. Incubation with antibody clone GHH8 (5μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with Im-mPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Chris Bonnas, Synaptic Systems, Göttingen, Germany).
B, Mouse liver after viral infection: Specific Detection of invading T-cells with monoclonal antibody clone GHH8 by IHC-FFPE. Note the clear membrane-bound signal free of background (left picture). Detection with a Ventana automated stainer following standard procedures. Incubation for 30min at 4°C with 10μg/ml of antibody clone GHH8, corresponding to a 1:50 dilution (Pic-ture courtesy of Susanne Krasemann, Mouse Pathology Core Facility, UKE, Hamburg, Germany).

A

B

References

1. Kersh AE et al. Cutting Edge: Resident Memory CD8 T Cells Express High-Affinity TCRs. Journal of Immunology 2015, 195: 3520-4.
2. Wu RC et al. New insights on the role of CD8(+)CD57(+) T-cells in cancer. Oncoimmunology 2012, 1: 954-56.
3. Hyland L et al. Mice lacking CD8+ T cells develop greater numbers of IgA-producing cells in response to a respiratory vi-rus infection. Virology 1994, 204: 234-41.
4. Prince HE et al. CD4 and CD8 subsets defined by dual-color cytofluorometry which distinguish symptomatic from asymp-tomatic blood donors seropositive for human immunodeficiency virus. Diagnostic & Clinical Immunology 1987, 5: 188-93.
5. Gaudernack G et al. Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies di-rectly conjugated to monosized magnetic microspheres. Journal of Immunological Methods 1986, 90: 179-87.
6. Fleischer B et al. Function of the CD4 and CD8 molecules on human cytotoxic T lymphocytes: regulation of T cell trigger-ing. Journal of Immunology 1986,136: 1625-8.

相关产品信息如下：

我们公司zui大优势是强大的采购，

1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，

2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，

3：提供加急服务，一般1-2周到货，超过时限加急费全免

4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是,因为意味着没有服务.

5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大 交大 复旦，中山等100多所大学，ROCHE，阿斯利康，国药 ，fisher等500多家公司

6：我们还是Santa,Advanced Biotechnologies Inc, Dianova,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. Dako,Inc, GePromegaridege,Dianova  Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,Dianova , Polysciences,prospecbi, QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司授权代理。
7：我们还是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批发，欢迎合作。