ESI BIO的使命是成为客户信赖的合作伙伴,将科学发现转化为诊所。我们通过提供突破性的干细胞技术作为研究级和治疗级产品来实现这一目标,从研究到诊所的照明,以帮助我们的客户实现他们治疗疾病的目标。
ESI BIO建立在丰富的创新和科学的基础之上。我们于2000年在新加坡成立了ES Cell International,在那里我们通过制造个可翻译的临床级胚胎干细胞系来创建历史,以便分发给研究界。
ESI BIO现在开展业务Alameda CA. 我们是BioTime,Inc。拥有的以干细胞为基础的子公司的骄傲家族的成员,我们从中受益于的科学,知识产权和干细胞技术的深层来源。
Product Name | SKU | Price |
HyStem®-C Hydrogel Trial Kit, 2.5 mL | GS312 | $85.00 |
HyStem®-C Hydrogel Trial Kit w/ PEGSSDA, 2.5 mL | GS312P | $125.00 |
HyStem®-C Hydrogel Kit, 7.5 mL | GS313 | $195.00 |
HyStem®-C Hydrogel Kit w/ PEGSSDA, 7.5 mL | GS313P | $225.00 |
HyStem®-C Hydrogel Kit, 12.5 mL | GS1005 | $245.00 |
HyStem ® -C水凝胶为用于细胞培养基质优化提供一个很好的起点。HyStem-C*化学成分确定的和基于三个生物相容性组件:巯基修饰的透明质酸(Glycosil),巯基反应性交联剂,PEGDA(Extralink),以及巯基修饰的胶原变性(格林-S ®)。Gelin-S为各种原代细胞和细胞系提供了基本的细胞附着位点,因此被推荐作为贴壁细胞类型和细胞培养优化的理想底物。在某些情况下,可以通过添加ECM蛋白来进一步增强HyStem-C水凝胶以匹配天然信号。
特征
· 水凝胶适用于培养原代细胞,干细胞和细胞系。
· 细胞可以以任何形式包封或生长在水凝胶表面上,包括培养瓶,6-至384孔板或组织培养插入物。
· 通过操纵组分浓度和混合比,使用者可以容易地定制水凝胶以具有所需的硬度和凝胶化时间。
· 可定制的凝胶特性,包括凝胶时间和水凝胶硬度。
凝胶化
重组的HyStem-C组分在15至37℃保持液态。当交联剂,Extralink在水凝胶形成®(PEGDA)加入到Glycosil的混合物®(巯基修饰的透明质酸)和格林-S ®(巯基修饰的明胶)。在所有三种组分混合后约20分钟发生凝胶化。没有步骤取决于低温或低pH。用磷酸盐缓冲盐水(PBS)或细胞培养基稀释组分可以增加凝胶化时间。
3D细胞恢复矩阵
对于细胞回收至关重要的应用,替代交联剂PEGSSDA可用于所有HyStem,HyStem-C和HyStem-HP试剂盒。这种交联剂具有Extralink提供的相同优点,还具有易于还原的内部键的额外优点。这允许从水凝胶中快速,容易地回收单细胞或簇,用于RNA分析或流式细胞术等应用,而不是可影响细胞活力的慢酶法。鼓励研究人员与我们联系,以确定特定细胞类型或培养系统与PEGSSDA的相容性。
储存和稳定性 | 将Glycosil和Gelin-S在-20°C的原始样品瓶中保存长达一年。 |
技术文件 | GS312技术数据表 产品支持 HyStem Hydrogels常见问题解答 小册子 HyStem ®水凝胶 |
组件 | Extralink ® |
参考 | 新!Gaetani,R.,et al。(2015)心脏祖细胞在3D打印的明胶/透明质酸贴剂中的心外膜应用保留了心肌梗塞后的心脏功能。Biomaterials 61:339-348。PMID:17335875。Prestwich |
ESI BIO产品目录:
货号 | 品名 | 规格 | 品牌 |
GS310 | HyStem® Hydrogel Kit | 2.5 mL | ESIBIO |
GS311 | HyStem® Hydrogel Kit | 7.5 mL | ESIBIO |
GS1004 | HyStem® Hydrogel Kit | 12.5 mL | ESIBIO |
GS310P | HyStem® Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS311P | HyStem® Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS312 | HyStem®-C Hydrogel Kit | 2.5 mL | ESIBIO |
GS313 | HyStem®-C Hydrogel Kit | 7.5 mL | ESIBIO |
GS1005 | HyStem®-C Hydrogel Kit | 12.5 mL | ESIBIO |
GS312P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS313P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS314 | HyStem®-HP Hydrogel Kit | 2.5 mL | ESIBIO |
GS315 | HyStem®-HP Hydrogel Kit | 7.5 mL | ESIBIO |
GS1006 | HyStem®-HP Hydrogel Kit | 12.5 mL | ESIBIO |
GS314P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS315P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS1007 | HyStem® Hydrogel UV QuickSet Kit | 2.5 mL | ESIBIO |
GS1008 | HyStem® Hydrogel UV QuickSet Kit | 7.5 mL | ESIBIO |
GS450 | PEGgel Kit | 1 mL | ESIBIO |
GS240 | DG Water | 10 mL | ESIBIO |
GS241 | DG Water | 20 mL | ESIBIO |
GS3007 | Extralink® Vial | 0.5 mL | ESIBIO |
GS3006 | Extralink® Vial | 2.5 mL | ESIBIO |
GS3009 | Extralink® Lite Vial | 0.5 mL | ESIBIO |
GS3008 | Extralink® Lite Vial | 2.5 mL | ESIBIO |
5050 | Fibronectin | 1 mg | ESIBIO |
GS231 | Gelin-S® Thiol-modified Gelatin | 1 mL | ESIBIO |
GS230 | Gelin-S® Thiol-modified Gelatin | 5 mL | ESIBIO |
GS222 | Glycosil® Hyaluronic Acid | 1 mL | ESIBIO |
GS220 | Glycosil® Hyaluronic Acid | 5 mL | ESIBIO |
GS217 | Heprasil® Hyaluronic Acid | 1 mL | ESIBIO |
GS215 | Heprasil® Hyaluronic Acid | 5 mL | ESIBIO |
5010-D | Nutragen® Bovine Collagen | 50 mL | ESIBIO |
GS711 | PEGDA | 1 mL | ESIBIO |
GS700 | PEGDA | 1 g | ESIBIO |
GS705 | PEGDA | 5 g | ESIBIO |
GS755 | PEGSSDA | 0.5 mL | ESIBIO |
5020 | PEPTITE-2000® RGD Peptide | 5 mg | ESIBIO |
5005-B | PureCol® Collagen | 100 mL | ESIBIO |
5007-A | VitroCol® Collagen | 20 mL | ESIBIO |
5051 | Vitronectin | 0.1 mg | ESIBIO |
EM2203 | ExoSense™ CD63 Exosome ELISA Kit | 96 assays | ESIBIO |
EM2202 | VascuNet™ CoCulture Assay Kit | 1 Kit | ESIBIO |
ST11006 | BioLiteTM SSEA-1 (DyLight 488) anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11008 | BioLiteTM TRA-1-81 (DyLight 488) anti-Human Antibody | 100 µL | ESIBIO |
ST11023 | Nestin anti-Human Antibody | 100 µL | ESIBIO |
ST11003 | Oct4 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11001 | Sox2 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11013 | SSEA-1 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11014 | SSEA-3 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11015 | SSEA-4 anti-Human Antibody | 100 µL | ESIBIO |
ST11018 | TRA-1-60 (PE) anti-Human Antibody | 100 µL | ESIBIO |
ST11016 | TRA-1-60 anti-Human Antibody | 100 µL | ESIBIO |
ST11017 | TRA-1-81 anti-Human Antibody | 100 µL | ESIBIO |
ST12007 | LIF, Mouse Recombinant | 10 µg | ESIBIO |
ST12008 | LIF, Mouse Recombinant | 100 µg | ESIBIO |
ES-84 | PureStem® 4D20.8, NCr-fac Progenitor | ea | ESIBIO |
ES-98 | PureStem® E15, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-283 | PureStem® 7PEND24, NCr-fac Progenitor | ea | ESIBIO |
ES-278 | PureStem® 7SMOO32, NCr-fac Progenitor | ea | ESIBIO |
ES-250 | PureStem® SK11, NCr-fac Progenitor | ea | ESIBIO |
ES-268 | PureStem® MEL2, NCr-fac Progenitor | ea | ESIBIO |
ES-256 | PureStem® SM30, NCr-fac Progenitor | ea | ESIBIO |
ES-335 | PureStem® ES-335 Meso-latp Progenitor | ea | ESIBIO |
ES-100 | PureStem® E72 BETATROPHIN+ Progenitor | ea | ESIBIO |
ES-1001 | PureStem® ES-1001 GDF11+ Progenitor | ea | ESIBIO |
ES-184 | PureStem® EN7 Progenitor | ea | ESIBIO |
ES-101 | PureStem® ES-101 Progenitor | ea | ESIBIO |
ES-139 | PureStem® ES-139 Progenitor | ea | ESIBIO |
ES-154 | PureStem® ES-154 Progenitor | ea | ESIBIO |
ES-198 | PureStem® ES-198 Progenitor | ea | ESIBIO |
ES-199 | PureStem® ES-199 Progenitor | ea | ESIBIO |
ES-209 | PureStem® ES-209, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-210 | PureStem® ES-210, Ecto-ntu Progenitor | ea | ESIBIO |
ES-236 | PureStem® ES-236 Progenitor | ea | ESIBIO |
ES-170 | PureStem® E44 Progenitor | ea | ESIBIO |
ES-196 | PureStem® W10 Progenitor | ea | ESIBIO |
ES-194 | PureStem® Z11, Meso Progenitor | ea | ESIBIO |
EM-1001 | PureStem® Progenitor Growth Media, EPM k01 | 500 mL | ESIBIO |
EM-1002 | PureStem® Progenitor Growth Media, EPM k02 | 500 mL | ESIBIO |
EM-1003 | PureStem® Progenitor Growth Media, EPM k03 | 500 mL | ESIBIO |
EM-1004 | PureStem® Progenitor Growth Media, EPM k04 | 500 mL | ESIBIO |
EM-1005 | PureStem® Progenitor Growth Media, EPM k05 | 500 mL | ESIBIO |
EM-1006 | PureStem® Progenitor Growth Media, EPM k06 | 500 mL | ESIBIO |
EM-1007 | PureStem® Progenitor Growth Media, EPM k07 | 500 mL | ESIBIO |
EM-1008 | PureStem® Progenitor Growth Media, EPM k08 | 500 mL | ESIBIO |
EM-2002 | HyStem®-4D Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2007 | HyStem®-4D Differentiation Kit | 1 Kit | ESIBIO |
EM-2001 | PureStem® Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2006 | PureStem® Choroid Plexus Differentiation Kit | 1 Kit | ESIBIO |
EM-2003 | PureStem® Osteogenesis Differentiation Kit 01 | 1 Kit | ESIBIO |
EM-2004 | PureStem® Osteogenesis Differentiation Kit 02 | 1 Kit | ESIBIO |
ST10035 | LY411575 | 5 mg | ESIBIO |
ST10008 | PD0325901 | 2 mg | ESIBIO |
ST10009 | PD0325901 | 10 mg | ESIBIO |
ST10010 | PD0325901 | 415 µL, 10 mM | ESIBIO |
ST10034 | PD173074 | 2 mg | ESIBIO |
ST10021 | RepSox | 5 mg | ESIBIO |
ST10027 | RG108 | 5 mg | ESIBIO |
ST10024 | SB203580 | 2 mg | ESIBIO |
ST10012 | SB431542 | 5 mg | ESIBIO |
ST10013 | SB431542 | 10 mg | ESIBIO |
ST10014 | SB431542 | 1.3 mL, 10 mM | ESIBIO |
ST10025 | SP600125 | 5 mg | ESIBIO |
ST10015 | Thiazovivin | 2 mg | ESIBIO |
ST10017 | XAV939 | 2 mg | ESIBIO |
ST10018 | Y27632 | 2 mg | ESIBIO |
ST10019 | Y27632 | 10 mg | ESIBIO |
ST10020 | Y27632 | 625 µL, 10 mM | ESIBIO |
上海起发实验试剂有限公司是实验试剂一站式采购服务商
1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。
2:产品种类齐全,经营超过700多品牌,基本涵盖所有生物实验试剂耗材。
3:提供加急服务,货品一般1-2周到货。
4:富有竞争力的价格优势,绝大部分价格有优势。
5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知药企。
6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司授权代理。
7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发,欢迎合作。