Nanopsy™是由先进的分子工程纳米粒子Magvigen™和Myquvigen™实现的,能够有效地从蛋白质、细胞和核酸中捕获和识别分子信息。我们正在将nanopsy™开发成可在成百上千家医院部署的、自动化和FDA批准的临床仪器和试剂盒,将致命的癌症转变为可控制的疾病。
用链霉亲和素珠和生物素探针捕获DNA
Magvigen™、Myquvigen™和Maxvigen™纳米颗粒是多功能和可生物降解的磁性和/或荧光磁性纳米颗粒,在加利福尼亚大学、伯克利大学、斯坦福大学和NVIGEN经过18年的严格研究和开发后达到。
产品名称 | 应用 | 目录号 | 规格/价格(1ml, USD) |
MagVigen™ – Plasma DNA Capture Kit | 对血浆中的 cfDNA 的捕获 | K61003 | Sizes (Plasma) 1,000 ml $3,999.00 |
产品介绍: MagVigen™ Plasma DNA Capture 磁性纳米颗粒专为捕获血液中的cfDNA而设计,为科研, 分子检测工作者提供了一种简单、快速、的血液cfDNA提取方法。整个实验过程中无需离心或者过柱。DNA提取可以线性比例扩大,输入血浆范围为100 uL至10mL 。分离出的cfDNA 可以用Agilent Bioanalyzer 直接定量分析。
下游应用: PCR, DNA测序,二代测序技术(NGS), 以及测序文库构建。
产品特点:
实验例子:
Figure 1. cfDNA extraction yield and size from 1.5 ml plasma samples with (blue) and without (red) spiking in DNA. Calculated yield of spiking in DNA by subtraction of endogenous cfDNA from total DNA were 80% using picogreen assay and 91% with bioanalyzer quantification.
Figure 2. cfDNA extracted from 100 µl to 5 ml of plasma was quantified by pico-green analysis.
Figure 3. cfDNA extraction yield was quantified by pico-green analysis following DNA isolation with the MagVigen™ Plasma DNA Capture Kit and kit C.
Figure 4. Beta-globin level and GAPDH levels were estimated by PCR analysis.