genelink 40-5131-05说明书
产品目录号 40-5131-05
产品名称 LiCl RNA 沉淀溶液 (7.5M LiCl，50 mM EDTA pH 8.0)；50 mL
Size50 mL
DescriptionLiCl RNA 沉淀溶液 (7.5M LiCl，50 mM EDTA pH 8.0)；50 mLDo 不用于小于 300 个核苷酸的 RNA 沉淀。终浓度为 2.5M 的 LiCl 在不添加乙醇的情况下可有效沉淀大于 300 个核苷酸的 RNA，这种沉淀方法选择性沉淀 RNA，不能有效沉淀 DNA、蛋白质或碳水化合物。它是从 RNA 制剂中去除翻译或 cDNA 合成抑制剂的优选方法。它还提供了一种从体外转录反应中回收 RNA 的简单快速方法。在 4 °C 下以 12K rpm 离心 20 min，可定量回收低至 50 ng 的 RNA。
用途：2.5 M 氯化锂终浓度，不添加乙醇或异丙醇。
浓度≥100 ng/mL 时 rna 的常规沉淀。
Catalog Number
40-5131-05
Product Name
LiCl RNA Precipitation Solution (7.5M LiCl, 50 mM EDTA pH 8.0); 50 mL
Size
50 mL
Description
LiCl RNA Precipitation Solution (7.5M LiCl, 50 mM EDTA pH 8.0); 50 mL
Do not use for precipitation of RNA smaller than 300 nucleotides.
A final concentration of 2.5M LiCl is effective in precipitation of RNA larger than 300 nucleotides without the addition of
ethanol, this method of precipitation selectively precipitates RNA and does not efficiently precipitate DNA, protein or
carbohydrate. It is the method of choice for removing inhibitors of translation or cDNA synthesis from RNA preparations. It
also provides a simple rapid method for recovering RNA from in vitro transcription reactions. As little as 50 ng of RNA can be
quantitatively recovered by centrifugation at 12K rpm for 20 minutes at 4°C.
Usage: 2.5M Lithium Chloride final concentration without addition of ethanol or isopropanol. Routine precipitation of
RNA at Concentrations ≥100 ng/mL.
