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abbexa abx370872​说明书

 更新时间:2022-06-24 点击量:439

Abbexa提供和经销生命科学,制药和生物技术方面的产品。公司位于英国剑桥,产品类 别包括一抗、二抗、同型对照、蛋白质、酶联免疫 (ELISA)试剂盒和酶等生物试剂。公司与世界不同实验室合作,研发与生物医学研究市场相关的高质量经过检验并且价格合理的产品,以满足客户的需要。近8000种一抗,包括少见的抗体如鲑鱼朊病毒单抗、霍乱内毒素EtxB单抗、糖基化载脂蛋白-B单抗、子宫内膜抗原单抗等,多种信号通路研究相关的抗体,很多不同位点磷酸化蛋白的抗体等。


abbexa abx370872说明书



1,25-Dihydroxyvitamin D3 (Calcitriol) Antibody Pair

货号: abx370872



用于竞争性 ELISA 检测开发的骨化3醇抗体对。该抗体对包含:


零件5 × 96 次测试10 × 96 次测试
捕获抗体200微克400微克
生物素共轭竞争者50微克100微克
标准2微克10微克


请注意,不同批次之间的数量和浓度可能会有所不同。

建议将此抗体对与abx098959 抗体对支持试剂盒(竞争方法)一起使用。

目标1,25-二羟基维生素(骨化3醇)
反应性人类
测试应用酶联免疫吸附试验
推荐稀释用包被缓冲液将 Capture Antibody 稀释 125 倍。
用生物素共轭竞争对手稀释剂将生物素共轭竞争对手稀释 200 倍。


最佳稀释度/浓度应由最终用户确定。
形式液体(捕获抗体和生物素偶联的竞争对手)
重组用标准稀释剂重新配制标准。体积和标准浓度应由最终用户确定。
贮存分装并储存在 -20°C。避免重复冻融循环。
缓冲Capture Antibody 和 Biotin-Conjugated Competor 均含有 0.1% 叠氮化纳
测试范围123.5 皮克/毫升 - 10000 皮克/毫升
标准格式冻干
检测类型竞争的
捕获抗体宿主兔子
捕获抗体克隆多克隆
捕获抗体偶联未结合的
检测抗体偶联生物素
专注捕获抗体:0.5 mg/ml
Biotin-Conjugated 竞争对手:0.2 mg/ml
使用说明将所有组件置于室温 (18-25°C) 并在使用前短暂旋转或离心小瓶。应立即准备和使用工作溶液。

推荐程序:

  1. 使用包被缓冲液将 Capture Antibody 稀释至工作浓度。立即用稀释的 Capture Antibody(每孔 100 µl)包被 96 孔板。密封板并在 4 °C 下孵育过夜或在 37 °C 下孵育 2 小时

  2. 吸出孔并用洗涤缓冲液(每孔 350 µl)洗涤,浸泡 1-2 分钟。将板倒置并轻敲到吸水纸上,以除去液体。

  3. 用封闭缓冲液(每孔 200 µl)在 37 °C 下封闭板 1.5 小时。

  4. 重复步骤 2 中的抽吸/清洗过程。

  5. 将 50 µl 标准品或样品添加到适当的孔中,然后添加 50 µl 工作的 Biotin-Conjugated Competor。盖上封板剂并在 37 °C 下孵育 1 小时。

  6. 重复步骤 2 中的抽吸/清洗过程。

  7. 加入适当稀释的链霉亲和素 HRP(每孔 100 µl)。用新的封板剂盖住盘子,在 37°C 下孵育 30 分钟。

  8. 重复步骤 2 中的抽吸/清洗过程,共 5 次。

  9. 添加底物溶液(每孔 90 µl)。用新的封板机盖住盘子,在 37°C 下孵育 10-20 分钟。将盘子放在黑暗中,避免暴露在光线下。

  10. 添加终止液(每孔 50 µl)。轻敲板的侧面以确保*混合。

  11. 使用设置为 450 nm 的酶标仪立即测量吸光度。

可用性5-20 个工作日内发货。
笔记本产品仅供研究使用。






ModelCategoryName

abx000001Primary AntibodiesHistone H3K27AC Antibody

abx000002Primary AntibodiesHistone H3K4me1 Antibody

abx000003Primary AntibodiesHistone H3K4me2 Antibody

abx000004Primary AntibodiesHistone H3K4me3 Antibody

abx000005Primary AntibodiesHistone H3K9me1 Antibody

abx000006Primary AntibodiesHistone H3K9me2 Antibody

abx000007Primary AntibodiesHistone H3K9me3 Antibody

abx000008Primary AntibodiesHistone H3K27me1 Antibody

abx000009Primary AntibodiesHistone H3K27me2 Antibody

abx000010Primary AntibodiesHistone H3K27me3 Antibody

abx000011Primary AntibodiesHistone H3K36me1 Antibody

abx000012Primary AntibodiesHistone H3K36me2 Antibody

abx000013Primary AntibodiesHistone H3K36me3 Antibody

abx000014Primary AntibodiesHistone H3K79me1 Antibody

abx000015Primary AntibodiesHistone H3K79me2 Antibody

abx000016Primary AntibodiesHistone H3K79me3 Antibody

abx000017Primary AntibodiesHistone H4K20me1 Antibody

abx000018Primary AntibodiesHistone H4K20me2 Antibody

abx000019Primary AntibodiesHistone H4K20me3 Antibody

abx000020Primary AntibodiesHistone H3R2me2s Antibody

abx000021Primary AntibodiesHistone H3R8me2s Antibody

abx000022Primary AntibodiesHistone H3R26me2a Antibody

abx000023Primary AntibodiesHistone H4R3me2a Antibody

abx000024Primary AntibodiesHistone H3R17me2a Antibody

abx000025Primary AntibodiesHistone H3R17me1 Antibody

abx000026Primary AntibodiesHistone H3R17me2s Antibody

abx000027Primary AntibodiesHistone H3R26me2s Antibody

abx000028Primary AntibodiesHistone H3R2me1 Antibody

abx000029Primary AntibodiesHistone H3R2me2a Antibody

abx000030Primary AntibodiesHistone H3R8me1 Antibody

abx000031Primary AntibodiesHistone H3R8me2a Antibody

abx000033Primary AntibodiesHistone H4R3me2s Antibody

abx000034Primary AntibodiesHistone H3R26me1 Antibody

abx000035Primary AntibodiesDimethyl-Histone H3-K14 Antibody

abx000036Primary AntibodiesTrimethyl-Histone H3-K14 Antibody

abx000037Primary AntibodiesPan Dimethyl-lysine Antibody

abx000038Primary AntibodiesHistone H3(mono+di+methyl K79) Antibody

abx000039Primary AntibodiesTumor Necrosis Factor Receptor Superfamily Member 1A / CD120a (TNFRSF1A) Antibody

abx000040Primary AntibodiesHepatocyte Growth Factor Receptor (MET) Antibody

abx000042Primary AntibodiesTransforming Growth Factor Beta 1 (TGFB1) Antibody

abx000043Primary AntibodiesMethylenetetrahydrofolate Reductase (MTHFR) Antibody

abx000044Primary AntibodiesAlbumin (ALB) Antibody

abx000047Primary AntibodiesChemokine C-X-C-Motif Receptor 4 (CXCR4) Antibody

abx000061Primary AntibodiesApolipoprotein A-V (APOA5) Antibody

abx000063Primary AntibodiesMyeloid/lymphoid Or Mixed-Lineage Leukemia (MLL) Antibody

abx000064Primary AntibodiesBromodeoxyuridine (BrdU) Antibody

abx000067Primary AntibodiesHeat Shock Protein 70 (HSP70) Antibody

abx000082Primary AntibodiesCadherin-1 / E-Cadherin (CDH1) Antibody

abx000083Primary AntibodiesCrystallin Beta B2 (CRYBB2) Antibody

abx000084Primary AntibodiesCaudal Type Homeobox 1 (CDX1) Antibody

abx000091Primary AntibodiesABL1 (pY245) Antibody

abx000093Primary AntibodiesABL1 (pY204) Antibody

abx000094Primary AntibodiesRAC-Alpha Serine/threonine-Protein Kinase Phospho-Thr450 (AKT1 pT450) Antibody

abx000095Primary AntibodiesRAC-Beta Serine/Threonine-Protein Kinase Phospho-Ser474 (AKT2 pS474) Antibody

abx000096Primary AntibodiesAPP (pT743) Antibody

abx000097Primary AntibodiesFAS (pY291) Antibody

abx000098Primary AntibodiesATM (pS1981) Antibody

abx000099Primary AntibodiesBad (pS118) Antibody

abx000100Primary AntibodiesBad (pS75) Antibody

abx000101Primary AntibodiesBad (pS99) Antibody

abx000102Primary AntibodiesG1/S-Specific Cyclin-E1 Phospho-Thr395 (CCNE1 pT395) Antibody

abx000104Primary AntibodiesCDK1 (pT14) Antibody

abx000105Primary AntibodiesCDK1 (pY15) Antibody

abx000106Primary AntibodiesCHEK1 (pS280) Antibody

abx000107Primary AntibodiesCHEK1 (pS317) Antibody

abx000108Primary AntibodiesCREB (pS133) Antibody

abx000109Primary AntibodiesATF2 (pT53) Antibody

abx000110Primary AntibodiesDnmt1 (phospho-pS714) Antibody

abx000111Primary AntibodiesDnmt1 (phospho-pY399) Antibody

abx000112Primary AntibodiesEGFR (pY869) Antibody

abx000113Primary AntibodiesEGFR (pY998) Antibody

abx000114Primary AntibodiesEGFR (pT693) Antibody

abx000115Primary AntibodiesEGFR (pY1016) Antibody

abx000116Primary AntibodiesEGFR (pY1172) Antibody

abx000117Primary AntibodiesEIF4EBP1 (pS65) Antibody

abx000118Primary AntibodiesELK1 (pS383) Antibody

abx000119Primary AntibodiesHER4 (pY1284) Antibody

abx000120Primary AntibodiesFGF Receptor 1 (pY766) Antibody

abx000121Primary AntibodiesFGF Receptor 1 (pY653) Antibody

abx000122Primary AntibodiesFGF Receptor 1 (pY654) Antibody

abx000123Primary AntibodiesProto-Oncogene C-Fos (pT232) (FOS) Antibody

abx000124Primary AntibodiesGSK3 (pS9) Antibody

abx000125Primary AntibodiesHSPB1 (pS15) Antibody

abx000126Primary AntibodiesHSPB1 (pS82) Antibody

abx000127Primary AntibodiesInsulin Receptor beta (pY1361) Antibody

abx000128Primary AntibodiesINSR (pY1146) / IGF1R (pY1131) Antibody

abx000129Primary AntibodiesINSR (pY1150) / IGF1R (pY1135) Antibody

abx000130Primary AntibodiesINSR (pY1151) / IGF1R (pY1136) Antibody

abx000131Primary AntibodiesInsulin Receptor beta (pY1355) Antibody

abx000132Primary Antibodiesc-Jun (pS73) Antibody

abx000133Primary Antibodiesc-Jun (pS63) Antibody

abx000134Primary Antibodiesc-Jun (pT239) Antibody

abx000135Primary Antibodiesc-Jun (pS243) Antibody

abx000136Primary AntibodiesERK3 (pS189) Antibody

abx000137Primary AntibodiesMAPK14 (pY322) Antibody

abx000138Primary AntibodiesMAPK14 (pT180) Antibody

abx000139Primary AntibodiesMAPK14 (pY182) Antibody

abx000140Primary AntibodiesMAP2K1 (pS217) Antibody

abx000141Primary AntibodiesMAP2K1 (pS297) Antibody

abx000142Primary AntibodiesMAP2K1 (phospho-pS221) Antibody

abx000143Primary AntibodiesMAP2K3 (pS189) Antibody

abx000144Primary AntibodiesSignal Transducer and Activator of Transcription 3 Phospho-Tyr705 (STAT3 pY705) Antibody

abx000145Primary AntibodiesMAX (pS11) Antibody

abx000146Primary AntibodiesMDM2 (pS166) Antibody

abx000147Primary AntibodiesMDM2 (pS260) Antibody

abx000148Primary AntibodiesMEF2C (pS396) Antibody

abx000149Primary AntibodiesMET (pY1003) Antibody

abx000150Primary AntibodiesMET (pY1349) Antibody

abx000151Primary AntibodiesMET (pY1234) Antibody

abx000152Primary AntibodiesMET (pY1235) Antibody

abx000153Primary AntibodiesMYC (pT58) Antibody

abx000154Primary AntibodiesMAP2K6 (pS207) Antibody

abx000155Primary AntibodiesMYC (pS62) Antibody

abx000156Primary Antibodiesp53 Phospho-Ser15 (p53 pS15) Antibody

abx000157Primary AntibodiesTP53 (pS9) Antibody

abx000158Primary AntibodiesRAC1 (pS71) Antibody

abx000159Primary AntibodiesRAF1 (pS621) Antibody

abx000160Primary AntibodiesRetinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody

abx000161Primary AntibodiesRetinoblastoma Protein 1 Phospho-Ser811 (RB1 pS811) Antibody

abx000162Primary AntibodiesSMC1A (pS957) Antibody

abx000163Primary AntibodiesHIST1H3B (pT12) Antibody

abx000164Primary AntibodiesMTOR (pS2448) Antibody

abx000165Primary AntibodiesHIST1H3B (pS29) Antibody

abx000166Primary AntibodiesRAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) Antibody

abx000167Primary AntibodiesHistone H2A.x (pS139) Antibody

abx000168Primary AntibodiesBtk (pY223) Antibody

abx000170Primary AntibodiesAMPK alpha (pT172) Antibody



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